Respuestas ampliatorias a preguntas formuladas luego de la conferencia:
Método para medir cortisol
-For plasmatic cortisol dosage, ethyl acetate extrac-164 tion was performed. Briefly, 100 μL of plasma was added to 1000 μL of ethyl acetate.
This solution was homogenized and then centrifuged at 10,000 × g for 15 minutes at 4 °C. 166 Then 400 μl of the supernatant was transferred to a microtube (Axygen PRO 1.5 mL, ref. 167 MCT-150-C-BRA) that was kept within the laminar flow for 12 hours. After evaporation 168 of the solution, the microtube was stored at –20 °C.
Efecto del enlatado sobre la preñez